Synthesis and investigation of the 5-formylcytidine modified, anticodon stem and loop of the human mitochondrial tRNAMet
نویسندگان
چکیده
Human mitochondrial methionine transfer RNA (hmtRNA(Met)(CAU)) has a unique post-transcriptional modification, 5-formylcytidine, at the wobble position-34 (f(5)C(34)). The role of this modification in (hmtRNA(Met)(CAU)) for the decoding of AUA, as well as AUG, in both the peptidyl- and aminoacyl-sites of the ribosome in either chain initiation or chain elongation is still unknown. We report the first synthesis and analyses of the tRNA's anticodon stem and loop domain containing the 5-formylcytidine modification. The modification contributes to the tRNA's anticodon domain structure, thermodynamic properties and its ability to bind codons AUA and AUG in translational initiation and elongation.
منابع مشابه
Synthesis and investigation of the 5-formylcytidine modified, anticodon stem and loop of the human mitochondrial tRNA
Human mitochondrial methionine transfer RNA ðhmtRNA CAUÞ has a unique post-transcriptional modification, 5-formylcytidine, at the wobble position-34 (fC34). The role of this modification in hmtRNA Met CAU for the decoding of AUA, as well as AUG, in both the peptidyland aminoacyl-sites of the ribosome in either chain initiation or chain elongation is still unknown. We report the first synthesis ...
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Mitochondrial (mt) tRNA(Met) has the unusual modified nucleotide 5-formylcytidine (f(5)C) in the first position of the anticodon. This tRNA must translate both AUG and AUA as methionine. By constructing an in vitro translation system from bovine liver mitochondria, we examined the decoding properties of the native mt tRNA(Met) carrying f(5)C in the anticodon compared to a transcript that lacks ...
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متن کاملUnconventional decoding of the AUA codon as methionine by mitochondrial tRNA with the anticodon fCAU as revealed with a mitochondrial in vitro translation system
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